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Kleinfelter's syndrome can be identified before birth by chorionic villus testing (cvt). The tests would only before preformed if there was a known history of genetic disorders in the family. 

If the syndrome is not detected at birth, it may go unnoticed until the adult male goes in to ask his doctor about infertility. To determine if a patient has Kleinfelter's syndrome, a chromosome anylysis if preformed. This karyotype is created by sampling the patient's peripheral blood. The blood must then be separated to sort out the lymphocytes and to see the chromosomal make up. If the karotype shows two X chromosomes and a single Y, then the

patient has Kleinfelter's syndrome.^[1]

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(Blood being drawin for a chromosomal analysis)